Ethanol extraction requirement for purification of protein labeled with [h]leucine in aquatic bacterial production studies.
نویسندگان
چکیده
The trichloroacetic acid (TCA)-insoluble fraction of water column bacteria labeled with [H]leucine contained an ethanol-soluble fraction accounting for up to 44% of the label. A component of the ethanol-soluble fraction is [H]leucine. Labeled-protein purification requires an ethanol wash step. Cold TCA can replace hot TCA for precipitation of labeled proteins.
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 54 12 شماره
صفحات -
تاریخ انتشار 1988